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Daphnia magna acute lethality toxicity test protocol

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Title: Daphnia magna acute lethality toxicity test protocol
Author: Poirier, D. G.; Westlake, G. F.; Abernethy, S. G.
Abstract: Daphnia magna (Straus) is a small planktonic crustacean which is widely distributed in ponds and lakes in Ontario, and is representative of filter-feeding zooplankton communities. This organism has been used in toxicity testing for over 100 years. As a test organism Daphnia has the advantages of being small (therefore only small amounts of effluent are required for each test), they are generally very sensitive, respond quickly to toxicants, and they are easily reared in large numbers In the laboratory. Proper culture conditions consist of moderately hard water (120 - 250 mg/L), with a pH between 6.5 and 8.5, and maintained at 20EC. Light intensity should not exceed 800 Lux at the water surface, and should follow a 16h:8h light:dark photoperiod. The toxicity test is a 48h static, acute test which uses only healthy neonate (1st Instar) D. magna less than 24h old. The test chambers can be any reasonable size, but must be constructed of glass and have a surface area to volume ratio (+ 10%) similar to that of a 25 x 150 mm test tube. Loading densities must not exceed 1 neonate per 15ml of test solution, and a minimum of ten Daphnia per concentration is required. Dissolved oxygen, pH and conductivity must be measured for each test concentration before and after the test. Estimation of the LC50 values, 95% confidence intervals, and slope of the probit line can be calculated by any acceptable method with preference to probit analysis using the maximum likelihood method, where data permits. All data must be supplied as per the "toxicity test summary report sheet" in appendix three.
Date: 1988
Rights: Queen's Printer for Ontario, Crown Copyright, Non-Commercial Use Permitted
Rights Holder: Queen's Printer for Ontario

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