Main content

Characterization of cardiac actin gene editing in zebrafish using CRISPR-Cas9 technology

Show full item record

Title: Characterization of cardiac actin gene editing in zebrafish using CRISPR-Cas9 technology
Author: Sandhu, Love
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Dawson, John
Abstract: Cardiomyopathy is a common cause of heart failure. Two prevalent forms of cardiomyopathy are hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM), characterized by changes to the myocardium. The development of HCM and DCM has been associated with mutations found in genes encoding muscle proteins, including cardiac actin (ACTC1). My research focused on the development of zebrafish as an in vivo model to study the underlying molecular mechanisms leading to cardiomyopathy development. Three zfactc genes have been identified through literature and phylogenetic analysis: zfactc1a, cardiofunk (zfacta1b), and zfactc1c. To validate zfactc genes as cardiac-specific, I used CRISPR-Cas9 to characterize editing these genes in zebrafish embryos. Research about the role of cardiac actin in cardiomyopathy development using zebrafish is translatable to humans because zebrafish and human ACTC proteins share 99 % sequence identity. This work will determine the roles of zfactc genes in cardiac development and for future human ACTC rescue experiments.
URI: http://hdl.handle.net/10214/15070
Date: 2019-02
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View
Sandhu_Love_201902_Msc.pdf 1.374Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record