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Staufen 1 does not play a role in NPC asymmetric divisions but regulates cellular positioning during corticogenesis

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Title: Staufen 1 does not play a role in NPC asymmetric divisions but regulates cellular positioning during corticogenesis
Author: Kuc, Christopher
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Vessey, John
Abstract: Cerebral cortex development relies on asymmetric divisions of neural precursor cells (NPCs) to produce a recurring NPC and a differentiated neuron. Asymmetric divisions are promoted by the differential localization of cell fate determinants between daughter cells. Staufen 1 (Stau1) is an RNA-binding protein known to localize mRNA in mature hippocampal neurons. However, its expression pattern and role in the developing mammalian cortex remains unknown. In this study, Stau1 mRNA and protein were found to be expressed in all cells examined and was temporally and spatially characterized across development. Upon shRNA-mediated knockdown of Stau1 in primary cortical cultures, NPCs retained the ability to self-renew and generate neurons despite the loss of Stau1 expression. This said, in vivo knockdown of Stau1 demonstrated that it may play a role in anchoring NPCs to the ventricular zone during cortical development.
URI: http://hdl.handle.net/10214/14756
Date: 2018-09


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