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Mechanisms of Glucocorticoid-Mediated Suppression of Tracheal Antimicrobial Peptide Expression in Bovine Tracheal Epithelial Cells

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dc.contributor.advisor Caswell, Jeff
dc.contributor.author Siracusa, Laura
dc.date.accessioned 2019-01-07T21:41:02Z
dc.date.available 2019-01-07T21:41:02Z
dc.date.copyright 2018-12
dc.date.created 2018-12-19
dc.date.issued 2019-01-07
dc.identifier.uri http://hdl.handle.net/10214/14742
dc.description.abstract This research aimed to identify the mechanism by which glucocorticoid downregulates inducible innate immune responses in bovine tracheal epithelial cells (bTEC). It adds knowledge regarding the bTEC surface receptor profile, as TLR 2 surface expression on bTEC was not identified. For this reason, the effect of glucocorticoid on expression of TLR 2 could not be determined. This research adds to the scant information available about the tracheal antimicrobial peptide (TAP) promoter region. The 500 bp sequence upstream of the TAP start site responded to inflammatory stimuli, but the 500, 1000 and 1500 bp upstream sequences had no observed response to glucocorticoid. Glucocorticoid upregulated IkB mRNA expression in bTEC, with no effect on other NF-κB-related genes. Thus, these studies identify reduced IkB gene expression as a likely mechanism by which glucocorticoid dampens this inducible innate immune response. These findings have significance in developing interventions to prevent stress-associated bovine respiratory disease. en_US
dc.description.sponsorship NSERC, Zoetis, the Ontario Ministry of Agriculture, Food and Rural Affairs. en_US
dc.language.iso en en_US
dc.rights Attribution 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by/4.0/ *
dc.subject Pathobiology en_US
dc.subject Molecular Biology en_US
dc.subject tracheal antimicrobial peptide en_US
dc.subject dual luciferase en_US
dc.subject NF-kB en_US
dc.subject mRNA en_US
dc.subject IkB en_US
dc.subject Inflammation en_US
dc.subject stress en_US
dc.subject glucocorticoid en_US
dc.subject cloning en_US
dc.subject toll-like receptor en_US
dc.subject TLR 2 en_US
dc.title Mechanisms of Glucocorticoid-Mediated Suppression of Tracheal Antimicrobial Peptide Expression in Bovine Tracheal Epithelial Cells en_US
dc.type Thesis en_US
dc.degree.programme Pathobiology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Pathobiology en_US
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Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International