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Mechanisms of Glucocorticoid-Mediated Suppression of Tracheal Antimicrobial Peptide Expression in Bovine Tracheal Epithelial Cells

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Title: Mechanisms of Glucocorticoid-Mediated Suppression of Tracheal Antimicrobial Peptide Expression in Bovine Tracheal Epithelial Cells
Author: Siracusa, Laura
Department: Department of Pathobiology
Program: Pathobiology
Advisor: Caswell, Jeff
Abstract: This research aimed to identify the mechanism by which glucocorticoid downregulates inducible innate immune responses in bovine tracheal epithelial cells (bTEC). It adds knowledge regarding the bTEC surface receptor profile, as TLR 2 surface expression on bTEC was not identified. For this reason, the effect of glucocorticoid on expression of TLR 2 could not be determined. This research adds to the scant information available about the tracheal antimicrobial peptide (TAP) promoter region. The 500 bp sequence upstream of the TAP start site responded to inflammatory stimuli, but the 500, 1000 and 1500 bp upstream sequences had no observed response to glucocorticoid. Glucocorticoid upregulated IkB mRNA expression in bTEC, with no effect on other NF-κB-related genes. Thus, these studies identify reduced IkB gene expression as a likely mechanism by which glucocorticoid dampens this inducible innate immune response. These findings have significance in developing interventions to prevent stress-associated bovine respiratory disease.
URI: http://hdl.handle.net/10214/14742
Date: 2018-12
Rights: Attribution 4.0 International
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Attribution 4.0 International Except where otherwise noted, this item's license is described as Attribution 4.0 International