Main content

Induction of the Persister Dormant State in Shiga Toxin-Producing Escherichia coli by Agents Encountered in the Soil Environment

Show simple item record

dc.contributor.advisor Warriner, Keith
dc.contributor.advisor Odumeru, Joseph
dc.contributor.author Tremblay, Chelsey
dc.date.accessioned 2019-01-07T21:05:32Z
dc.date.available 2019-01-07T21:05:32Z
dc.date.copyright 2018-12
dc.date.created 2018-12-12
dc.date.issued 2019-01-07
dc.identifier.uri http://hdl.handle.net/10214/14741
dc.description.abstract Persisters refer to a subpopulation of cells that enter a dormant state despite conditions being favorable for growth. By entering a dormant state cells exhibit high stress tolerance, in addition to antibiotic resistance. The study illustrated that STEC serotypes exhibit a range of abilities to enter into the persister state and that indole may play a role in regulating transition into the dormant state. Studies focused on agents encountered in the field environment that could induce the persister state within STEC to explain the extended persistence of the pathogens within soil. It was found that E. coli O103 produced a greater proportion of persisters in comparison to both O157 and P36, a generic E. coli strain. The inclusion of 500μM of indole increased the number of persisters formed by E. coli O103 compared to controls. Other agents tested did not statistically produce more persisters compared to controls although levels were highly variable between replicates. The study illustrated that STEC serotypes exhibit a range of abilities to enter into the persister state and that indole may play a role in regulating transition into the dormant state. en_US
dc.description.sponsorship Center for Produce Safety en_US
dc.language.iso en en_US
dc.subject STEC en_US
dc.subject Escherichia coli en_US
dc.subject persister en_US
dc.subject indole en_US
dc.subject bile en_US
dc.title Induction of the Persister Dormant State in Shiga Toxin-Producing Escherichia coli by Agents Encountered in the Soil Environment en_US
dc.type Thesis en_US
dc.degree.programme Food Science en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Food Science en_US
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View
Tremblay_Chelsey_201901_Msc.pdf 1.894Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record