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Polyisoprenoid Alcohols Influence Plastidial Membrane Dynamics and Photosynthetic Performance in Solanum lycopersicum and Arabidopsis thaliana

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dc.contributor.advisor Akhtar, Tariq
dc.contributor.author Virta, Lilia
dc.date.accessioned 2018-11-02T14:31:49Z
dc.date.available 2018-11-02T14:31:49Z
dc.date.copyright 2018
dc.date.created 2018-10-30
dc.date.issued 2018-11-02
dc.identifier.uri http://hdl.handle.net/10214/14365
dc.description.abstract Polyisoprenoid alcohols (polyprenols and dolichols) are a class of natural products whose distribution throughout the plant kingdom is well known, yet their functional role is poorly understood. These compounds are assembled by a class of enzymes known as cis-prenyltransferases (CPTs), which are encoded by small gene families in plants. In this study, RNAi-mediated knockdown of members of the tomato (Solanum lycopersicum) and Arabidopsis thaliana CPT families (SlCPT5 and AtCPT7) reduced polyprenols in leaves by ~60%. Subcellular fractionation studies and in vivo localization of CPT fluorescent protein fusions demonstrated that both CPTs reside in the chloroplast stroma and their products accumulate into chloroplast membranes. Fluorescence anisotropy measurements revealed that polyprenol-deficient chloroplast membranes were more disordered than wildtype membranes. These ‘disordered’ membranes exhibited lower phase transition temperatures and calorimetric enthalpies relative to wildtype, indicating alterations in integral membrane protein stability. These results uncover a role for polyprenols in governing chloroplast membrane dynamics. en_US
dc.description.sponsorship NSERC, Ontario Ministry of Agriculture Food and Rural Affairs en_US
dc.language.iso en en_US
dc.rights Attribution-NoDerivatives 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by-nd/4.0/ *
dc.subject cis-prenyltransferase en_US
dc.subject polyprenol en_US
dc.subject membrane dynamics en_US
dc.subject photosynthetic performance en_US
dc.title Polyisoprenoid Alcohols Influence Plastidial Membrane Dynamics and Photosynthetic Performance in Solanum lycopersicum and Arabidopsis thaliana en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
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