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Polyisoprenoid Alcohols Influence Plastidial Membrane Dynamics and Photosynthetic Performance in Solanum lycopersicum and Arabidopsis thaliana

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Title: Polyisoprenoid Alcohols Influence Plastidial Membrane Dynamics and Photosynthetic Performance in Solanum lycopersicum and Arabidopsis thaliana
Author: Virta, Lilia
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Akhtar, Tariq
Abstract: Polyisoprenoid alcohols (polyprenols and dolichols) are a class of natural products whose distribution throughout the plant kingdom is well known, yet their functional role is poorly understood. These compounds are assembled by a class of enzymes known as cis-prenyltransferases (CPTs), which are encoded by small gene families in plants. In this study, RNAi-mediated knockdown of members of the tomato (Solanum lycopersicum) and Arabidopsis thaliana CPT families (SlCPT5 and AtCPT7) reduced polyprenols in leaves by ~60%. Subcellular fractionation studies and in vivo localization of CPT fluorescent protein fusions demonstrated that both CPTs reside in the chloroplast stroma and their products accumulate into chloroplast membranes. Fluorescence anisotropy measurements revealed that polyprenol-deficient chloroplast membranes were more disordered than wildtype membranes. These ‘disordered’ membranes exhibited lower phase transition temperatures and calorimetric enthalpies relative to wildtype, indicating alterations in integral membrane protein stability. These results uncover a role for polyprenols in governing chloroplast membrane dynamics.
URI: http://hdl.handle.net/10214/14365
Date: 2018
Rights: Attribution-NoDerivatives 4.0 International


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Attribution-NoDerivatives 4.0 International Except where otherwise noted, this item's license is described as Attribution-NoDerivatives 4.0 International