Main content

Flavonol catabolism in Arabidopsis thaliana: subcellular localization of a flavonol 3-O-glucoside beta-glucosidase and biochemical evidence for a flavonol rhamnoside 7-O-alpha-rhamnosidase

Show simple item record

dc.contributor.advisor Bozzo, Gale
dc.contributor.author Gordon, Harley O. W.
dc.date.accessioned 2018-10-22T14:50:12Z
dc.date.available 2019-10-17T05:00:34Z
dc.date.copyright 2018-10
dc.date.created 2018-10-17
dc.date.issued 2018-10-22
dc.identifier.uri http://hdl.handle.net/10214/14361
dc.description.abstract Flavonols are specialized plant metabolites with numerous human health promoting properties. Flavonol glycosides accumulate in Arabidopsis in response to abiotic stresses, but their concentrations decrease with stress recovery. Moreover, the catabolism of flavonol 3-O-β-glucosides is dependent upon activity of the β-glucosidase BGLU15. Transient expression of a BGLU15-mCherry fusion protein in onion epithelial cells revealed that it is localized to the apoplast; this implies flavonol glucoside catabolism is an extracellular process. In Arabidopsis, the predominant glycosyl modification of flavonols is rhamnosylation at their 7-hydroxy position. High-performance liquid chromatography analysis of enzyme assays containing cell-free Arabidopsis leaf extracts revealed the presence of flavonol 7-O-α-rhamnosidase activity. This activity increased in Arabidopsis leaves as they aged. Minor levels of this activity were present in roots, whereas no activity was detected in stems. The identification of this novel flavonol 7-O-α-rhamnosidase activity may have future implications for biotechnological strategies aimed at enhancing flavonol levels in plants. en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 International *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.subject Flavonol en_US
dc.subject Plant metabolism en_US
dc.subject Arabidopsis thaliana en_US
dc.subject Flavonol catabolism en_US
dc.title Flavonol catabolism in Arabidopsis thaliana: subcellular localization of a flavonol 3-O-glucoside beta-glucosidase and biochemical evidence for a flavonol rhamnoside 7-O-alpha-rhamnosidase en_US
dc.type Thesis en_US
dc.degree.programme Plant Agriculture en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Plant Agriculture en_US
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View Description
Gordon_Harley_201810_Msc.pdf 1.755Mb PDF View/Open Harley Gordon MSc Thesis - resubmission

This item appears in the following Collection(s)

Show simple item record

Attribution-NonCommercial-NoDerivatives 4.0 International Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivatives 4.0 International