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Storage and Propagation Characteristics of Miscanthus x giganteus

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Title: Storage and Propagation Characteristics of Miscanthus x giganteus
Author: Downey, Cassandra
Department: Department of Plant Agriculture
Program: Plant Agriculture
Advisor: Jones, Andrew Maxwell Phineas
Abstract: Miscanthus x giganteus Anderss. exhibits favourable characteristics as a dedicated second-generation biomass feedstock. Propagation of this sterile species occurs mainly through micropropagation or rhizome cuttings, resulting in high establishment costs. This thesis sought to investigate the improvement of storage duration for field-harvested rhizomes, prolongment of callus regeneration potential through impairment of the phenylpropanoid biosynthetic pathway, and the induction of microrhizomes through the modification of media compositions. It was found that plant growth from rhizomes yielded similar results after spring and autumn harvests, plantlet regeneration was achieved from calli after 12 months in culture when media was supplemented with 2-aminoindan-2-phosphonic acid, and microrhizomes were successfully induced over a 10-week incubation period in liquid media supplemented with 8% sucrose, 6-benzylaminopurine, and 1-naphthaleneacetic acid. These findings may be used to improve conservation, reduce establishment costs, and allow for a greater supply of Miscanthus biomass in the marketplace.
Date: 2018-09
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