Main content

Resistance to fungicides in the plant pathogen Microdochium nivale

Show full item record

Title: Resistance to fungicides in the plant pathogen Microdochium nivale
Author: Gourlie, Ryan
Department: School of Environmental Sciences
Program: Environmental Sciences
Advisor: Hsiang, Tom
Abstract: Samples of Microdochium nivale were collected from British Columbia and Ontario. The sampled isolates (161) and previously collected isolates (129) were tested for fungicide sensitivity by growing on amended media. Iprodione and propiconazole EC50 values ranged from 1 to 543 μg mL-1¬ and 0.001 to 0.87 μg mL-1 respectively. In field trials, resistance was significantly correlated with less disease. Draft genome assemblies of 13 isolates were searched for sequences associated with resistance. Alignments of the mnos-1 gene revealed a mutation (A3503G) and a deletion (Δ430-462) in iprodione-resistant isolates. Mutations in mnos-4 (G49A and C396T), and mutations in skn7 (214 SNPs, six deletions, two insertions) were associated with a subset of iprodione-resistant isolates. Mutations in the mrr1 gene (G2237A and G2392A) were associated with resistance to both fungicides in some isolates. No mutations in the propiconazole target site (cyp51) were found to be associated with propiconazole-resistant isolates of M. nivale.
Date: 2018-09
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.

Files in this item

Files Size Format View Description
Gourlie_Ryan_201809_MSc.pdf 3.556Mb PDF View/Open MSc thesis

This item appears in the following Collection(s)

Show full item record