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Adaptation of Green Proteorhodopsin to Changes in Membrane Lipid Composition

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dc.contributor.advisor Ladizhansky, Vladimir
dc.contributor.advisor Brown, Leonid
dc.contributor.author Brown, Rachel Ellen
dc.date.accessioned 2018-09-05T17:41:44Z
dc.date.available 2018-09-05T17:41:44Z
dc.date.copyright 2018-08
dc.date.created 2018-08-31
dc.date.issued 2018-09-05
dc.identifier.uri http://hdl.handle.net/10214/14219
dc.description.abstract The protein studied in this thesis is Proteorhodopsin (PR), which is linked to starvation states in marine bacteria. This work examines PR membrane system stability using FTIR. The stable membrane systems, found using FTIR, are further examined using multiple methodologies. The findings show lipid loss over time for PR membrane systems that contained only PC lipids or a DOPC/DOPS mixture. Lipid systems containing DOPE/DOPG were found to be stable and were compared to systems containing DMPC/DMPA in further experiments. These systems mimic the changes involved in the transition from non-starvation to starvation state, suggesting DMPC/DMPA as a suitable native membrane model for structural study of PR. Photocycle and titration experiments provide evidence of a shift in the pKa of D97 (DMPC/DMPA PR 6.6, DOPE/DOPG PR 7.5). CANCO experiments showed minimal changes in observable residues, however further NMR studies are required for full evaluation. en_US
dc.language.iso en en_US
dc.subject Proteorhodopsin en_US
dc.subject Membrane Properties en_US
dc.subject NMR en_US
dc.subject FTIR en_US
dc.title Adaptation of Green Proteorhodopsin to Changes in Membrane Lipid Composition en_US
dc.type Thesis en_US
dc.degree.programme Biophysics en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Physics en_US
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