Main content

Investigating skeletal muscle passive mechanical properties: the effects of fibre type, muscle, age, and species

Show full item record

Title: Investigating skeletal muscle passive mechanical properties: the effects of fibre type, muscle, age, and species
Author: Noonan, Alex
Department: Department of Human Health and Nutritional Sciences
Program: Human Health and Nutritional Sciences
Advisor: Brown, Stephen
Abstract: Currently, there is a gap in the literature regarding information on the passive mechanical properties of the various fibre and muscle types, as well as how passive muscle mechanical properties change with age and differ across species. As such, passive muscle stiffness (elastic modulus) of single muscle fibres were investigated in the current thesis. The purpose of this thesis was to: investigate what effect fibre type (type 1 versus type 2) had on passive elastic moduli; to compare passive elastic moduli amongst the rat hindlimb muscles (Tibialis Anterior (TA), Soleus (SOL), and Gastrocnemius (GAST)); to compare passive elastic moduli in young and old humans. Single muscle fibres were placed in a relaxing solution and repeatedly stretched to calculate the elastic modulus. Fibre types were determined via gel electrophoresis (SDS-PAGE). Type 1 fibres were stiffer then type 2 fibres; however when accounting for fibre diameter, this difference no longer existed. There was no difference in stiffness amongst the hindlimb muscles of the rat. “Young” single fibres were stiffer then “old” single fibres. Single fibres from humans were stiffer than single fibres from the rat hindlimb. Together these data improve the current understanding of passive muscle properties of the various fibre and muscle types, as well as how passive muscle properties change with age and differ across species.
URI: http://hdl.handle.net/10214/14204
Date: 2018
Terms of Use: All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View
Noonan_Alex_201808_MSc.pdf 2.036Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record