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Structural Influence of Hemicyanine Dyes in Optical Detection Platforms

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Title: Structural Influence of Hemicyanine Dyes in Optical Detection Platforms
Author: Chung, Andrew
Department: Department of Chemistry
Program: Chemistry
Advisor: Manderville, Richard
Abstract: Hemicyanine dyes are highly conjugated fluorescent molecules that are composed of a D-π-A motif that exhibits the ability to push electrons from the donor towards the acceptor, generating emission and excitation wavelengths in the visible region. The research discussed in this thesis will demonstrate the utility of hemicyanine dyes for detection of thiolate anions and ochratoxin A. First, two hemicyanine dyes with commonly employed A moieties undergo nucleophilic addition with a simple thiolate to provide unambiguous 1H and 13C NMR evidence to distinguish the products of solvolysis from thiolate addition. For detection of OTA, various hemicyanine dyes are shown to bind to the ochratoxin A aptamer to produce a turn-on emission response. Addition of the toxin to the dye:aptamer complex resulted in loss of dye emission. For these studies, the nature of the A plays a critical role in the ability of OTA to displace the dye from the aptamer.
Date: 2018-08-22
Rights: Attribution 2.5 Canada
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Attribution 2.5 Canada Except where otherwise noted, this item's license is described as Attribution 2.5 Canada