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Investigating Foodborne Cryptosporidium and Giardia in Clams Harvested Near Iqaluit, Nunavut

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dc.contributor.advisor Shapiro, Karen
dc.contributor.advisor Harper, Sherilee
dc.contributor.author Manore, Anna Jane Wilson
dc.date.accessioned 2018-08-23T16:16:34Z
dc.date.available 2018-08-23T16:16:34Z
dc.date.copyright 2018-08
dc.date.created 2018-07-18
dc.date.issued 2018-08-23
dc.identifier.uri http://hdl.handle.net/10214/14091
dc.description.abstract High prevalences of Cryptosporidium and Giardia were reported in enteric illness patients in Nunavut, Canada, with a foodborne, waterborne, or animal source of parasites suspected. Clams (Mya truncata) are a commonly consumed, culturally important, and nutritious country food in Iqaluit, Nunavut; however, shellfish may concentrate pathogens from surrounding waters. EcoHealth approaches were used to investigate locally-harvested clams as a potential source of Cryptosporidium and Giardia for Iqaluit residents. Following molecular method validation, clam hemolymph (n=328) and digestive gland (n=390) samples were screened for Cryptosporidium and Giardia via PCR followed by sequence analyses. Giardia was confirmed in hemolymph from 2 clams, while Cryptosporidium was not detected. The Giardia sequences were identified as zoonotic Giardia enterica assemblage B, and may have originated from human or animal sources. Study results are intended to inform public health practice and planning. en_US
dc.description.sponsorship ArcticNet, Ontario Graduate Scholarship, Ontario Veterinary College Student Stipend, Canadian Institutes of Health Research Canada Graduate Scholarship, the Arthur D. Latornell Travel Grant, Northern Scientific Training Program, Michael Smith Foreign Study Supplement en_US
dc.language.iso en en_US
dc.subject Cryptosporidium en_US
dc.subject Giardia en_US
dc.subject Toxoplasma en_US
dc.subject Clams en_US
dc.subject Mya truncata en_US
dc.subject Canada en_US
dc.subject Arctic en_US
dc.subject Nunavut en_US
dc.subject Country Food en_US
dc.subject Inuit Health en_US
dc.subject EcoHealth Approaches en_US
dc.subject DNA Extraction en_US
dc.subject Freeze-Thaw en_US
dc.subject PCR en_US
dc.title Investigating Foodborne Cryptosporidium and Giardia in Clams Harvested Near Iqaluit, Nunavut en_US
dc.type Thesis en_US
dc.degree.programme Population Medicine en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Population Medicine en_US
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