Main content

The sex-dependent interplay of integrin alpha1beta1 and epidermal growth factor receptor in regulating oxidative stress in articular cartilage

Show full item record

Title: The sex-dependent interplay of integrin alpha1beta1 and epidermal growth factor receptor in regulating oxidative stress in articular cartilage
Author: Haskins, James
Department: Department of Human Health and Nutritional Sciences
Program: Human Health and Nutritional Sciences
Advisor: Clark, Andrea
Abstract: The collagen receptor integrin alpha1beta1 slows cartilage degradation in mouse models of osteoarthritis. Epidermal growth factor receptor (EGFR) signalling appears to affect multiple aspects of cartilage health, although whether integrin alpha1beta1 regulates EGFR and downstream superoxide production has not been demonstrated in chondrocytes. We examined how integrin alpha1beta1/EGFR interplay regulates superoxide levels in ex vivo articular chondrocytes of male and female mice. Through confocal microscopy, we determined that chondrocytes of male mice had elevated levels of superoxide compared to female mice. Only male mice demonstrated a condyle effect, with greater superoxide levels in the medial condyles, whereas female mice showed a genotype effect, whereby integrin alpha1beta1 restricted superoxide abundance. Inhibition of EGFR with erlotinib dampened superoxide levels in the medial condyles of male mice but exacerbated superoxide abundance in wild-type female mice. These findings indicate a need for the consideration of sex and compartment when assessing cell signalling in cartilage.
URI: http://hdl.handle.net/10214/14072
Date: 2018-08
Rights: Attribution-NoDerivs 2.5 Canada


Files in this item

Files Size Format View Description
Haskins_James_201808_Msc.pdf 51.25Mb PDF View/Open Thesis

This item appears in the following Collection(s)

Show full item record

Attribution-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NoDerivs 2.5 Canada