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Evaluation of the impact of azo dyes on the metabolism of stabilized fecal communities and in vitro cell culture

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Title: Evaluation of the impact of azo dyes on the metabolism of stabilized fecal communities and in vitro cell culture
Author: Ivusic Polic, Ives
Department: Department of Food Science
Program: Food Science
Advisor: LaPointe, Gisèle
Abstract: The human gut microbiota is a complex and dynamic ecosystem of microbes existing in symbiosis with the host and can be altered by diet. Azo dyes are present in a large portion of our diet. To investigate the impact of azo dyes on gut microbial metabolites, a stabilized fecal slurry was subjected to Tartrazine exposure and metabolites were analyzed via 1-dimensional proton nuclear magnetic resonance. Results revealed that Tartrazine had a negative effect on 10 out of 13 profiled metabolites. Tartrazine had a negative impact on the transepithelial resistance of in vitro cultured Caco2 epithelial cells and increased the secretion of TNFα. Data from Guelph Health Family Studies suggested that children up to 6 years of age tend to consume 1.2 meals daily containing azo dye. This study suggests that dyes present in food interact with gut microbiota; the resulting metabolites may cause inflammation, leading to effects on human health.
URI: http://hdl.handle.net/10214/13525
Date: 2018-05
Rights: Attribution-NoDerivs 2.5 Canada


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Attribution-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NoDerivs 2.5 Canada
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