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An Investigation of the Metabolic Health Benefits of Purple Vegetables in an Animal Model of Metabolic Syndrome Using Molecular Approaches

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dc.contributor.advisor Meckling, Kelly Ayoub, Hala 2018-05-24T19:13:35Z 2018-05-24T19:13:35Z 2018-05 2018-05-18 2018-05-24
dc.description.abstract This thesis is an investigation of the benefits of purple vegetables on metabolic health. Consumption of anthocyanin rich plants is positively associated with improvements in various Metabolic Syndrome (MetS) parameters. Purple potatoes (PP) and purple carrots (PC) have higher anthocyanin concentrations and higher biological activity than lightly pigmented cultivars. However, they have not been extensively studied for their potential metabolic health benefits. I hypothesized that the substitution of the majority of carbohydrate in a high fat diet, with PC or PP in obese Zucker rats will 1) A) Improve insulin resistance and hypertension, two main components of MetS, compared to lightly colored varieties of these same vegetables (white potatoes (WP) and orange carrots (OC)) and a sucrose rich diet. B) All the vegetable diets will be superior to the sucrose rich diet. 2) Underlying mechanisms will include modulating adipose and hepatic gene expression. PP and WP consumption improved insulin sensitivity compared to the sucrose rich diet where PP were slightly superior to WP. The two carrot groups showed similar trends to the potatoes but this did not reach statistical significance. Each of the potatoes and the carrots groups improved blood pressure compared to the control group. However, each of the WP and PC were superior to their corresponding color counterpart. Using Western blotting, there were no obvious differences in the expression of a group of the candidate proteins (e.g. FAS, ACC, AMPK, PPAR γ and adiponectin) chosen for the initial mechanistic investigation. Nonetheless, using proteomic analyses, we were able to identify few biological themes modulated by each vegetable in both tissues. We proposed that enhanced protein folding, increased cholesterol efflux and higher free fatty acids re-esterification are mechanisms that participate in the action of PP and PC on MetS pathologies in adipose tissue whereas decreased de novo lipogenesis and oxidative stress to be the suggested mechanisms of action in liver. In conclusion, evidence is provided at both the phenotypic and the molecular level, for the metabolic benefits of purple vegetables. Inclusion of these vegetables in the human diet is suggested as a strategy to improve MetS associated pathologies. en_US
dc.description.sponsorship OMAFRA en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri *
dc.subject polyphenols en_US
dc.subject flavonoids en_US
dc.subject diabetes en_US
dc.subject Cardiovascular disease en_US
dc.subject hypertension en_US
dc.subject insulin resistance en_US
dc.subject high fat diet en_US
dc.subject purple carrots and potatoes en_US
dc.subject proteomic analyses en_US
dc.title An Investigation of the Metabolic Health Benefits of Purple Vegetables in an Animal Model of Metabolic Syndrome Using Molecular Approaches en_US
dc.type Thesis en_US Human Health and Nutritional Sciences en_US Doctor of Philosophy en_US Department of Human Health and Nutritional Sciences en_US
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Ayoub_Hala_201805_PhD.pdf 2.213Mb PDF View/Open Ayoub Hala PhD thesis
Supplemental Ta ... eins in Adipose tissue.csv 158.1Kb Unknown View/Open Supplemental Table 1
Supplemental Ta ... nd pathways_PP_Adipose.csv 36.55Kb Unknown View/Open Supplemental Table 2
Supplemental Ta ... fied proteins in liver.csv 75.00Kb Unknown View/Open Supplemental Table 3
Supplemental Ta ... and pathways_PP_Liver.csv 4.293Kb Unknown View/Open Supplemental Table 4
Supplemental Ta ... nd pathways_PC_Adipose.csv 114.8Kb Unknown View/Open Supplemental Table 5
Supplemental Ta ... and pathways_PC_liver.csv 25.69Kb Unknown View/Open Supplemental Table 6

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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada