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Investigating a role for phospholipids in plastid pleomorphy

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dc.contributor.advisor Mathur, Jaideep Anderson, Cole 2018-05-09T20:53:48Z 2019-04-19T05:00:26Z 2018-04 2018-04-19 2018-05-09
dc.description.abstract Plastids, a defining feature of plants, produce stroma-filled extensions or tubules known as stromules. Although stromules are reliably induced upon exogenous sucrose treatment and inhibited upon silver nitrate treatment, a clear mechanism and function behind this phenomenon remains to be elucidated. The lack of inorganic phosphate appears to affect stromule levels as well as simultaneously cause a conversion of extraplastidic membrane lipids from phospholipids to galactolipids, suggesting a lipid-based mechanism for their formation. Non-specific phospholipase C 4 and non-specific phospholipase C 5, while responsible for this conversion, do not affect stromule formation. The origin of plastidial phosphatidylcholine upstream of phospholipid to galactolipid conversion is likely due to the presence of plastid associated membranes and does not play a role in stromule formation. The observations from my microscopy based studies demonstrate the organelle pleomorphy influencing ability of lysophosphatidylcholine, implicating acyl-editing, better known as the Lands cycle, in stromule formation. en_US
dc.description.sponsorship Natural Sciences and Engineering Research Council of Canada en_US
dc.language.iso en en_US
dc.publisher University of Guelph en_US
dc.subject plastid en_US
dc.subject chloroplast en_US
dc.subject PLA2 en_US
dc.subject phospholipase A2 en_US
dc.subject PC en_US
dc.subject phosphatidylcholine en_US
dc.subject LPC en_US
dc.subject lysophosphatidylcholine en_US
dc.subject LPCAT en_US
dc.subject lysophosphatidylcholine acyltransferase en_US
dc.title Investigating a role for phospholipids in plastid pleomorphy en_US
dc.type Thesis en_US Molecular and Cellular Biology en_US Master of Science en_US Department of Molecular and Cellular Biology en_US
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated. University of Guelph en_US

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