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Investigation and Optimization of Methods for the Extraction of Antioxidants and Polyphenols from Yellow European Plums (Prunus Domestica L.)

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dc.contributor.advisor Singh, Ashutosh
dc.contributor.author DiNardo, Andrea
dc.date.accessioned 2018-05-01T20:44:13Z
dc.date.available 2018-05-01T20:44:13Z
dc.date.copyright 2018-05
dc.date.created 2018-04-12
dc.date.issued 2018-05-01
dc.identifier.uri http://hdl.handle.net/10214/12950
dc.description.abstract Yellow European Plums (YEPs) contain health promoting compounds which may be extracted for use in functional food and nutraceutical industries. Conventional extraction methods include heat reflux extraction (HRE) and Soxhlet extraction, but often result in reduced extraction yields due to high temperature requirements and long extraction durations. Microwave-assisted extraction (MAE) is one method which can mitigate these drawbacks because it allows rapid and homogenous heating the biomatrix. The objective of this thesis is to determine the effects of solvent and solvent concentration, time, and temperature during MAE in comparison to other extraction methods. Concentration of phenolic compounds, specifically ascorbic acid, chlorogenic acid, neochlorogenic acid, were quantified using a Folin-Ciocalteau method and HPLC analysis. Antioxidant scavenging of free radicals such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Fe3+-tripyridyltraizine (FRAP) were used to determine antioxidant capacity of samples. It was observed that MAE yielded larger concentrations of bioactive compounds in comparison to other extraction methods. en_US
dc.description.sponsorship Natural Sciences and Engineering Research Council, Arrell Food Institute at the University of Guelph, Food from Thought Initiative, and Ontario Ministry of Agriculture, Food and Rural Affairs. en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject Yellow European Plums en_US
dc.subject microwave-assisted extraction en_US
dc.subject phenolics en_US
dc.subject antioxidants en_US
dc.title Investigation and Optimization of Methods for the Extraction of Antioxidants and Polyphenols from Yellow European Plums (Prunus Domestica L.) en_US
dc.type Thesis en_US
dc.degree.programme Engineering en_US
dc.degree.name Master of Applied Science en_US
dc.degree.department School of Engineering en_US
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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada