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Isolation and Characterization of Bacteriophages against Listeria monocytogenes and their Applications as Biosensors for Foodborne Pathogen Detection

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Title: Isolation and Characterization of Bacteriophages against Listeria monocytogenes and their Applications as Biosensors for Foodborne Pathogen Detection
Author: Fallatah, Safaa
Department: Department of Food Science
Program: Food Science
Advisor: Mansel, Griffiths
Abstract: The aim of this study was to determine the potential application of bacteriophages for the detection of Listeria spp. on food contact surfaces (FCS). Eight phages were selected for further characterization to determine the most appropriate phage for use in a detection assay. They were characterized for host range, TEM, stability to air dying for 24 h at 25 °C, and restriction endonuclease pattern. L. monocytogenes strain C716 was very resistant to all phages, however; a mutated phage, AG13M, was able to infect L. monocytogenes strain C716. AG20 & AG23 phages were high specificity against their host (Listeria spp). AG20 phage was immobilized on ColorLok paper and used in the to detect L. monocytogenes C519 in broth and FCS. AG20 phage was able to detect as few as 50 CFU/mL of L. monocytogenes in TSB and 40 CFU/cm2 on FCS using a plaque assay to detect progeny phage within 24 h.
URI: http://hdl.handle.net/10214/12614
Date: 2018-02
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