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Zebrafish upregulate cortisol catabolism as a neuroprotective coping mechanism in response to waterborne cortisol exposure

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dc.contributor.advisor Bernier, Nicholas
dc.contributor.author Bernards, Mark
dc.date.accessioned 2018-04-17T12:52:54Z
dc.date.available 2019-03-26T05:00:29Z
dc.date.copyright 2018-03
dc.date.created 2018-03-26
dc.date.issued 2018-04-17
dc.identifier.uri http://hdl.handle.net/10214/12609
dc.description.abstract The biology of chronic stress is a subject of interest due to its proposed negative consequences, including deficits to neurological welfare. To isolate the effects of chronic stress-induced elevated cortisol on neurogenesis, we developed a system that continuously exposed zebrafish to exogenous cortisol. We found that waterborne cortisol elicits small, transient increases in neurogenesis-related genes in the forebrain but no sustained effects. Though whole-body cortisol did not change, the exposure regime resulted in rapid, dose-dependent, and sustained increases in the forebrain gene expression of cortisol catabolic enzymes and whole-body levels of an inactive cortisol metabolite 20-dihydrocortisone. Differences in corticosteroid receptor expression – sequential, transient increase and decrease in both gr and mr and delayed, sustained decrease in gr – suggest differential involvement in the stress response. Overall, we provide a basis for developing a temporal map of chronic stress in zebrafish and highlight their impressive capacity for cortisol catabolism and neuroprotection in response to exogenous cortisol. en_US
dc.language.iso en en_US
dc.subject zebrafish en_US
dc.subject cortisol en_US
dc.subject neurogenesis en_US
dc.subject stress en_US
dc.subject hydroxysteroid dehydrogenase en_US
dc.subject 20beta-dihydrocortisone en_US
dc.title Zebrafish upregulate cortisol catabolism as a neuroprotective coping mechanism in response to waterborne cortisol exposure en_US
dc.type Thesis en_US
dc.degree.programme Integrative Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Integrative Biology en_US


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