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Characterizing the hnRNP Q Complex and its Activity in Asymmetric Neural Precursor Cell Divisions during Cerebral Cortex Development

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dc.contributor.advisor Vessey, John
dc.contributor.author Smart, Anastasia
dc.date.accessioned 2018-02-01T19:35:34Z
dc.date.available 2019-01-26T06:00:17Z
dc.date.copyright 2018-01
dc.date.created 2018-01-26
dc.date.issued 2018-02-01
dc.identifier.uri http://hdl.handle.net/10214/12543
dc.description.abstract The development of the cerebral cortex is reliant upon the collaborative efforts of a wide variety of molecular mechanisms, one of which is the ability of neural precursor cells (NPCs) to undergo asymmetric cell division. Asymmetric cell divisions produce two daughter cells with distinct identities, one NPC and one neuron. The identity of each daughter cell is decided by the unequal distribution of cell fate determinants. This imbalance is created by the partitioning of RNA:protein complexes to an intracellular site for localization. As a result, the post-mitotic translation of localized transcripts dictates the relative abundance of cell fate determinants and thus, daughter cell identity. Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a family of RNA-binding proteins with diverse functions. This research aimed to characterize the hnRNP Q complex and its activity in asymmetric NPC division. The postnatal expression profile revealed hnRNP Q expression persists beyond embryonic development into the immediate postnatal period. Inhibiting nuclear export in NPCs and isolating the hnRNP Q complex via co-immunoprecipitation indicated hnRNP Q shuttles in and out of the nucleus and is capable of forming nuclear and cytoplasmic complexes, suggesting hnRNP Q is involved in splicing, localization and translational repression. Finally, in vivo knockdown experiments replicated in vitro findings that revealed altered patterns of NPC division that show a bias towards self-renewal. en_US
dc.language.iso en en_US
dc.title Characterizing the hnRNP Q Complex and its Activity in Asymmetric Neural Precursor Cell Divisions during Cerebral Cortex Development en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
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