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In vitro Evaluation of the Effects of Combining Bisphosphonates and Radiation Therapy in Canine Osteosarcoma Cells

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Title: In vitro Evaluation of the Effects of Combining Bisphosphonates and Radiation Therapy in Canine Osteosarcoma Cells
Author: Hoddinott, Katie
Department: Department of Clinical Studies
Program: Veterinary Science
Advisor: Oblak, MichelleMutsaers, Anthony
Abstract: Osteosarcoma (OSA) continues to be a devastating disease in veterinary medicine. One palliative treatment option currently being employed is the combination of radiation therapy and bisphosphonates despite a limited body of literature to support its use. Investigation of this combined therapy has been performed in human and murine OSA cells in vitro, however this is the first report of their evaluation in canine OSA cells in vitro. Dose dependent effects of pamidronate, zoledronate and radiation were identified in two canine OSA cell lines: D17 and Dharma. Some combinations of bisphosphonates and radiation resulted in decreased clonogenic survival and cell viability compared to single agent therapies. Evaluation of the combined effects of these two modalities via combination index identified antagonistic relationships between pamidronate + radiation and zoledronate + radiation. Furthermore, timing of administration of bisphosphonates in relation to radiation indicated post-radiation treatment with bisphosphonates resulted in an increased response to treatment under our laboratory conditions.
URI: http://hdl.handle.net/10214/12159
Date: 2017-12


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