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rDNA Copy Number Change and Sequence Variation in Mutation Accumulation Lines of Daphnia pulex

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dc.contributor.advisor Crease, Teresa
dc.contributor.author Harvey, Eleanor Floy
dc.date.accessioned 2018-01-05T19:40:04Z
dc.date.available 2018-01-05T19:40:04Z
dc.date.copyright 2018-01
dc.date.created 2017-12-15
dc.date.issued 2018-01-05
dc.identifier.uri http://hdl.handle.net/10214/12156
dc.description.abstract Freshwater ecosystems are subject to a variety of stressors, including metal contamination. Lakes and ponds in southern Ontario are especially contaminated with metals, as mines in this region are among the top copper and nickel producers in the world. These metals can mutate DNA, although it is generally unknown how the freshwater microcrustacean Daphnia pulex is affected by chronic metal exposure. To determine how metal exposure affects D. pulex ribosomal (r)DNA, a highly conserved genetic compartment, I estimated rDNA copy number and sequence variation in mutation accumulation lines of D. pulex. I found that chronic metal exposure results in large fluctuations in rDNA copy number in D. pulex, including significant losses of rDNA copies. However, chronic metal stress does not influence the rate of new point mutations in D. pulex rDNA. These findings contribute to our understanding of how D. pulex rDNA evolves with chronic, sub-lethal exposure to metal. en_US
dc.description.sponsorship This project was supported in part by an ERASMUS grant, an NSERC CREATE grant for Aquatic Ecosystem Health created by Dr. Melania Cristescu. en_US
dc.language.iso en en_US
dc.rights Attribution-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nd/2.5/ca/ *
dc.title rDNA Copy Number Change and Sequence Variation in Mutation Accumulation Lines of Daphnia pulex en_US
dc.type Thesis en_US
dc.degree.programme Integrative Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Integrative Biology en_US
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Attribution-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NoDerivs 2.5 Canada