Main content

rDNA Copy Number Change and Sequence Variation in Mutation Accumulation Lines of Daphnia pulex

Show simple item record

dc.contributor.advisor Crease, Teresa Harvey, Eleanor Floy 2018-01-05T19:40:04Z 2018-01-05T19:40:04Z 2018-01 2017-12-15 2018-01-05
dc.description.abstract Freshwater ecosystems are subject to a variety of stressors, including metal contamination. Lakes and ponds in southern Ontario are especially contaminated with metals, as mines in this region are among the top copper and nickel producers in the world. These metals can mutate DNA, although it is generally unknown how the freshwater microcrustacean Daphnia pulex is affected by chronic metal exposure. To determine how metal exposure affects D. pulex ribosomal (r)DNA, a highly conserved genetic compartment, I estimated rDNA copy number and sequence variation in mutation accumulation lines of D. pulex. I found that chronic metal exposure results in large fluctuations in rDNA copy number in D. pulex, including significant losses of rDNA copies. However, chronic metal stress does not influence the rate of new point mutations in D. pulex rDNA. These findings contribute to our understanding of how D. pulex rDNA evolves with chronic, sub-lethal exposure to metal. en_US
dc.description.sponsorship This project was supported in part by an ERASMUS grant, an NSERC CREATE grant for Aquatic Ecosystem Health created by Dr. Melania Cristescu. en_US
dc.language.iso en en_US
dc.rights Attribution-NoDerivs 2.5 Canada *
dc.rights.uri *
dc.title rDNA Copy Number Change and Sequence Variation in Mutation Accumulation Lines of Daphnia pulex en_US
dc.type Thesis en_US Integrative Biology en_US Master of Science en_US Department of Integrative Biology en_US
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.

Files in this item

Files Size Format View Description
Harvey_Eleanor_201801_MSc.pdf 3.626Mb PDF View/Open Main manuscript

This item appears in the following Collection(s)

Show simple item record

Attribution-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NoDerivs 2.5 Canada