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Investigating the use of anaerobic fungi to enhance the hydrolysis of lignocellulose in lab scale biodigesters

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dc.contributor.advisor Gilroyed, Brandon Ravi, Bhargavi 2018-01-05T14:22:42Z 2018-01-05T14:22:42Z 2017-12 2017-12-01 2018-01-05
dc.description.abstract This thesis investigated hydrolysis of lignocellulosic biomass using bioaugmentation with rumen isolated anaerobic fungi (AF). Hydrolysis of corn silage was compared with hydrolysis of common reed using three different genera of AF. Based on production of H2 and CO2, accumulation of volatile fatty acids and pH change, low levels of hydrolytic activity were observed in all treatments, possibly due to poor survival of AF in experimental bioreactors. The presence of background microflora during hydrolysis was a confounding factor in assessing the efficacy of AF. In a follow up study, AF were inoculated into sterilized corn silage to remove background microbial activity. In addition to the chemical parameters measured as markers of hydrolysis, hydrolytic enzyme activity was also assessed. However, no significant improvement in hydrolysis was observed regardless of the fungal genera used, again suggesting poor survival of AF in bioreactors. en_US
dc.description.sponsorship Natural Resources Canada Program of Energy Research and Development (grant number Inc001), Agriculture and Agri-Food Canada (grant number T.2002.36) and the Natural Sciences and Engineering Research Council of Canada (grant number 400806). en_US
dc.language.iso en en_US
dc.subject hydrolysis en_US
dc.subject pretreatment en_US
dc.subject anaerobic fungi en_US
dc.subject N. frontalis en_US
dc.subject P. rhizinflatus en_US
dc.subject A. mucronatus en_US
dc.subject corn silage en_US
dc.subject common reed en_US
dc.title Investigating the use of anaerobic fungi to enhance the hydrolysis of lignocellulose in lab scale biodigesters en_US
dc.type Thesis en_US Environmental Sciences en_US Master of Science en_US School of Environmental Sciences en_US
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