Main content

A molecular investigation of the interaction between Rubisco and beta-carboxysome interior organizer CcmM

Show simple item record

dc.contributor.advisor Kimber, Matthew
dc.contributor.author Ryan, Patrick
dc.date.accessioned 2017-12-22T18:20:54Z
dc.date.available 2017-12-22T18:20:54Z
dc.date.copyright 2017-10
dc.date.created 2017-12-20
dc.date.issued 2017-12-22
dc.identifier.uri http://hdl.handle.net/10214/12120
dc.description.abstract Cyanobacteria and some chemoautotrophs sequester Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) in close proximity to CO2 using large proteinaceous microcompartments called carboxysomes, to improve carbon fixation efficiency. Carboxysomes are built from tens of thousands of protein subunits with a closed icosahedral shell that is selectively permeable for certain substrates and metabolites. This shell encases functional enzymes, including Rubisco, within. CcmM is an interior β-carboxysome protein that has been implicated in organizing the carboxysome interior. The C-terminal domain of CcmM is built of three to five subunits that closely resemble the small subunit of Rubisco, and have been implicated in organizing Rubisco into a core that the carboxysomal shell forms around. This thesis reports the first known X-ray crystallographic structure of the first C-terminal domain in the chain “SS1” from T. elongatus BP-1. Förster Resonance Energy Transfer, Surface Plasmon Resonance and Native mass spectrometry experiments show CcmM binds Rubisco with micromolar affinities. Six SS1 domains can bind to Rubisco with no indication of RbcS release, suggesting that binding does not fully displace RbcS. A novel molecular binding model for the CcmM-Rubisco complex is proposed. en_US
dc.description.sponsorship Natural Sciences and Engineering Research Council en_US
dc.language.iso en en_US
dc.subject carboxysome en_US
dc.subject rubisco en_US
dc.subject ccmm en_US
dc.title A molecular investigation of the interaction between Rubisco and beta-carboxysome interior organizer CcmM en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
dc.rights.license All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.


Files in this item

Files Size Format View Description
ryan_patrick_201912_Msc.pdf 15.60Mb PDF View/Open Master's thesis

This item appears in the following Collection(s)

Show simple item record