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A53T alpha-synuclein induces mitophagy through MAM-induced fission and reduced impairment of LC3B to promote alpha-synuclein degradation

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Title: A53T alpha-synuclein induces mitophagy through MAM-induced fission and reduced impairment of LC3B to promote alpha-synuclein degradation
Author: Humphries, Kayla
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Ryan, Scott
Abstract: Parkinson’s disease (PD) is a neurodegenerative disorder characterized by mitochondrial dysfunction and aggregated α-synuclein (α-syn). Some cases of PD are caused by mutations in α-syn, resulting in an increased propensity for aggregation. To investigate the link between mitochondrial dysfunction and the α-syn mutation A53T, human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), each consisting of an A53T line and a wildtype or corrected control line, were differentiated into dopaminergic human neurons (hNs). Transmission electron microscopy demonstrated that A53T cells had elongated mitochondrial-associated membranes (MAMs) at early timepoints, which were shortened to normal lengths by day 60 of differentiation, concurrent with mitochondrial fragmentation. Golgi dilation and vacuolar area were increased in A53T hNs, indicative of an increased autophagic load. Immunofluorescence showed that aggregated α-syn has a greater colocalization with lysosomes in A53T hNs, suggesting lysosomes degrade, or attempt to degrade, aggregated α-syn. This degradation could occur alongside mitophagy, as knockdown of the apoptotic protein beclin1 decreased cell survival in the A53T line, indicating mitophagy is protective. Further, in vitro experiments demonstrate that A53T α-syn has a reduced ability to impair binding of the mitophagy-initiating protein LC3B to mitochondria, suggesting that A53T cells would be more vulnerable to mitophagy. Potential therapies should focus on preventing MAM-induced fission and restoring the ability of A53T cells to impair LC3B-mediated mitophagy.
Date: 2017-12
Rights: Attribution-NonCommercial-NoDerivs 2.5 Canada

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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada
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