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Autophagy-related gene expression in rainbow trout (Oncorhynchus mykiss) and their role in nutrient restriction in vivo and in vitro

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dc.contributor.advisor Lumsden, John S.
dc.contributor.author Balmori-Cedeno, Jaramar
dc.contributor.author Lumsden, John S.
dc.date.accessioned 2017-10-03T18:18:54Z
dc.date.available 2017-10-03T18:18:54Z
dc.date.copyright 2017-09
dc.date.created 2017-09-19
dc.date.issued 2017-10-03
dc.identifier.uri http://hdl.handle.net/10214/11620
dc.description.abstract The expression of ten autophagy-related genes (ATG4, ATG5, ATG7, ATG9, ATG12, ATG13, ATG16, BECN-1, GABARAP and LC3) was examined in RTgill-W1 (gill epithelial) cells and in muscle and liver from rainbow trout (Oncorhynchus mykiss) undergoing serum nutrient restriction and compared to control groups. Six of these sequences, ATG5, ATG7, ATG9, ATG13, ATG16 and BECN-1, were previously unknown in rainbow trout. The rainbow trout sequences had highest nucleotide sequence similarity with Atlantic salmon and other fish species used for comparison in this study. In serum-restricted RTgill-W1 cells, most autophagy-related genes were significantly expressed at Day 3. In liver from feed-restricted fish, most Atg were significantly upregulated within the first 4 days. Autophagy-related gene expression in muscle was significantly upregulated between Day 8 and 15. Both tissues had a second peak of Atg upregulation at Day 21 suggesting that autophagy is maintained for longer periods of time in fish than previously understood. en_US
dc.description.sponsorship NSERC OMAFRA OVC en_US
dc.language.iso en en_US
dc.subject autophagy en_US
dc.subject rainbow en_US
dc.subject trout en_US
dc.subject nutrient en_US
dc.subject restriction en_US
dc.subject Atg en_US
dc.subject genes en_US
dc.title Autophagy-related gene expression in rainbow trout (Oncorhynchus mykiss) and their role in nutrient restriction in vivo and in vitro en_US
dc.type Thesis en_US
dc.degree.programme Pathobiology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Pathobiology en_US
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