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Evaluation of Flea Beetle (Phyllotreta spp.) Resistance in Spring and Winter-type Canola (Brassica napus).

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dc.contributor.advisor Kott, Laima
dc.contributor.advisor Rajcan, Istvan Heath, Julian 2017-09-26T19:12:45Z 2017-09-26T19:12:45Z 2017-09 2017-07-25 2017-09-26
dc.description.abstract This thesis is an investigation into the understanding of flea beetle (Phyllotreta spp.) resistance in spring-type and winter-type canola quality Brassica napus L.. Canola is one of the world’s most widely grown oilseed crops and an economically important crop in Western Canada. Genetic resistance to this common pest would add to the available tactics for integrated pest management of flea beetles in canola. The purpose of this research was to better understand the interactions of the flea beetle with canola at key feeding times in the life cycle of the flea beetle and identify genetic components related to flea beetle herbivory on canola seedlings. The objectives were to: 1) investigate seasonal effects of flea beetle herbivory on both spring-type and winter-type canola; and 2) identify quantitative trait loci (QTL) for flea beetle herbivory in two winter-type doubled haploid (DH) populations using simple sequence repeat (SSR) markers and single nucleotide polymorphic (SNP) markers. Year and seasonal effects were noted but overall trends amongst entries were similar concluding that flea beetle feeding patterns did not change throughout its life cycle. Spring-type and winter-type germplasm reacted similarly under flea beetle feeding. As such, there does not appear to be any novel resistance mechanisms that evolved as a result of divergent growth habit types in canola. Seven QTL were identified over the iii two DH populations studied. Linkage group (LG) N13 had multiple QTL identified. The remaining QTL were located on LG N04, N06, N15 and N17. It is unknown as to what mechanisms these QTL are associated with. The results of this thesis provide insight into flea beetle-canola plant interactions and identify some genetic areas of interest related to flea beetle herbivory in canola. en_US
dc.description.sponsorship DuPont Pioneer en_US
dc.language.iso en en_US
dc.subject Brassica napus en_US
dc.subject canola en_US
dc.subject insect resistance en_US
dc.subject flea beetle resistance en_US
dc.subject flea beetle en_US
dc.subject Phyllotreta cruciferae en_US
dc.subject Phyllotreta striolata en_US
dc.subject SSR en_US
dc.subject SNP en_US
dc.subject linkage map en_US
dc.subject agriculture en_US
dc.subject plant breeding en_US
dc.title Evaluation of Flea Beetle (Phyllotreta spp.) Resistance in Spring and Winter-type Canola (Brassica napus). en_US
dc.type Thesis en_US Plant Agriculture en_US Doctor of Philosophy en_US Department of Plant Agriculture en_US
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