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Investigating the Independent Roles of Linoleic Acid and Arachidonic Acid on White Adipose Tissue Inflammation in Fads2 Knock-Out Mice

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dc.contributor.advisor Mutch, David
dc.contributor.author Suitor, Katherine
dc.date.accessioned 2017-09-06T18:34:49Z
dc.date.available 2017-09-06T18:34:49Z
dc.date.copyright 2017-08
dc.date.created 2017-08-31
dc.date.issued 2017-09-06
dc.identifier.uri http://hdl.handle.net/10214/11545
dc.description.abstract This thesis is an investigation into the influence of two distinct n-6 PUFA, linoleic acid (LA) and arachidonic acid (AA), on inflammatory signalling pathways in white adipose tissue (WAT) using fatty acid desaturase 2 knock-out (Fads2-/-) mice. Delineating the independent roles of n-6 PUFA is challenging due to the continuous endogenous conversion of LA into AA. Fads2 regulates the initial rate-limiting step in this conversion. Therefore, Fads2-/- mice are unable to convert LA into AA. Following consumption of carefully formulated low-fat diets for nine weeks, LA and AA were highly enriched in serum and different WAT depots in both wild-type and Fads2-/- mice; however, neither n-6 PUFA activated inflammatory gene and protein expression. Furthermore, the outcomes were similar in both subcutaneous and visceral adipose tissue depots. Collectively this thesis demonstrates that LA and AA do not induce WAT inflammation when consumed as part of a low fat diet. en_US
dc.description.sponsorship This work was supported by grants #450115 from the Canadian Institutes of Health Research and #371546 from the Natural Sciences and Engineering Research Council of Canada. en_US
dc.language.iso en en_US
dc.subject polyunsaturated fats en_US
dc.subject omega-6 en_US
dc.subject white adipose tissue en_US
dc.subject Fads2 en_US
dc.subject linoleic acid en_US
dc.subject arachidonic acid en_US
dc.title Investigating the Independent Roles of Linoleic Acid and Arachidonic Acid on White Adipose Tissue Inflammation in Fads2 Knock-Out Mice en_US
dc.type Article en_US
dc.type Thesis en_US
dc.degree.programme Human Health and Nutritional Sciences en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Human Health and Nutritional Sciences en_US
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