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Characterization of the Canine Immunoglobulin Heavy Chain Repertoire and Design of an Improved Molecular Clonality Assay for Canine B Cell Proliferations

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dc.contributor.advisor Keller, Stefan
dc.contributor.author Hwang, Mei-Hua
dc.date.accessioned 2017-09-06T17:51:19Z
dc.date.available 2018-08-28T05:00:38Z
dc.date.copyright 2017-08
dc.date.created 2017-08-28
dc.date.issued 2017-09-06
dc.identifier.uri http://hdl.handle.net/10214/11542
dc.description.abstract Molecular clonality assays for canine B cell proliferations have low sensitivity, which can lead to false negative results. This study aimed to characterize the canine immunoglobulin heavy chain repertoire and to design a novel assay with improved sensitivity. The immune repertoires of 3 healthy dogs were assessed for V/J gene usage and pairing biases, isotype usage, CDR3 diversity, convergent recombination, and shared clonotypes. An in silico assessment of existing primer sets identified commonly rearranged genes that were not covered by the assays. Revised primer sets were designed to reduce primer-template mismatches. When tested against the original assay, revised primer sets generally showed improved sensitivity and specificity. The improved primer sets enable more reliable and accurate diagnoses, which allow for prompt treatment and higher quality patient care and life. In addition, this study for the first time compared the performance of all major assays allowing for evidence-based selection of primer sets. en_US
dc.description.sponsorship OVC Pet Trust research grant, NSERC Discovery Grant, OVC scholarship, NSERC CSG-M program. en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject molecular clonality testing en_US
dc.subject PCR en_US
dc.subject canine immune repertoire en_US
dc.subject immunoglobulin heavy chain en_US
dc.subject b cell lymphoma en_US
dc.subject lymphocyte antigen receptor gene rearrangement en_US
dc.subject veterinary diagnostics en_US
dc.subject Research Subject Categories::VETERINARY MEDICINE en_US
dc.title Characterization of the Canine Immunoglobulin Heavy Chain Repertoire and Design of an Improved Molecular Clonality Assay for Canine B Cell Proliferations en_US
dc.type Thesis en_US
dc.degree.programme Pathobiology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Pathobiology en_US


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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada