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Characterization of the Canine Immunoglobulin Heavy Chain Repertoire and Design of an Improved Molecular Clonality Assay for Canine B Cell Proliferations

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Title: Characterization of the Canine Immunoglobulin Heavy Chain Repertoire and Design of an Improved Molecular Clonality Assay for Canine B Cell Proliferations
Author: Hwang, Mei-Hua
Department: Department of Pathobiology
Program: Pathobiology
Advisor: Keller, Stefan
Abstract: Molecular clonality assays for canine B cell proliferations have low sensitivity, which can lead to false negative results. This study aimed to characterize the canine immunoglobulin heavy chain repertoire and to design a novel assay with improved sensitivity. The immune repertoires of 3 healthy dogs were assessed for V/J gene usage and pairing biases, isotype usage, CDR3 diversity, convergent recombination, and shared clonotypes. An in silico assessment of existing primer sets identified commonly rearranged genes that were not covered by the assays. Revised primer sets were designed to reduce primer-template mismatches. When tested against the original assay, revised primer sets generally showed improved sensitivity and specificity. The improved primer sets enable more reliable and accurate diagnoses, which allow for prompt treatment and higher quality patient care and life. In addition, this study for the first time compared the performance of all major assays allowing for evidence-based selection of primer sets.
URI: http://hdl.handle.net/10214/11542
Date: 2017-08
Rights: Attribution-NonCommercial-NoDerivs 2.5 Canada


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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada