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Bioactivity of Bovine Colostrum and Milk Exosomes from High, Average, and Low Immune Responders on Human Intestinal Epithelial Cells

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dc.contributor.advisor Mallard, Bonnie
dc.contributor.author Ross, Mikayla
dc.date.accessioned 2017-09-06T16:28:23Z
dc.date.available 2018-08-29T05:00:18Z
dc.date.copyright 2017-08
dc.date.created 2017-08-29
dc.date.issued 2017-09-06
dc.identifier.uri http://hdl.handle.net/10214/11538
dc.description.abstract Bovine colostrum and milk contain bioactive components that are nutritionally and immunologically important to calves and humans. While high (H) immune responder cows have improved colostrum and milk quality compared to average (A) and low (L) responders, bioactivity of colostrum and milk exosomes containing microRNAs on human intestinal epithelial cells was unknown. Therefore, the objectives of this research were to evaluate intestinal epithelial cell uptake, viability, and caspase 3 activity after co-culture with colostrum and milk exosomes from H, A, and L immune responders. Results indicated exosomes were taken up by epithelial cells, enhance cell viability, and activate the caspase 3 pathway. Further, this research provides novel insights into differences in bioactivity between colostrum and milk exosomes from H, A, and L immune responders, whereby cell viability was significantly greater after co-culture with H responder exosomes compared to L. Collectively, these findings suggest potential health benefits of cow’s milk. en_US
dc.description.sponsorship Ontario Ministry of Agriculture, Food and Rural Affairs, Dairy Farmers of Ontario, and Natural Sciences and Engineering Research Council of Canada. en_US
dc.language.iso en en_US
dc.subject microRNA en_US
dc.subject human intestinal epithelial cells en_US
dc.subject High Immune Responders en_US
dc.subject Caco-2 cells en_US
dc.subject MTT en_US
dc.subject caspase 3 en_US
dc.subject cell viability en_US
dc.subject apoptosis en_US
dc.subject uptake assay en_US
dc.subject bovine colostrum and milk exosomes en_US
dc.title Bioactivity of Bovine Colostrum and Milk Exosomes from High, Average, and Low Immune Responders on Human Intestinal Epithelial Cells en_US
dc.type Thesis en_US
dc.degree.programme Pathobiology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Pathobiology en_US
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