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Production of protegrin-1 with a matrix metalloproteinase/elastase cleavage site and its therapeutic potential for skin wound infections

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dc.contributor.advisor Li, Julang
dc.contributor.author Hill, Emily
dc.date.accessioned 2017-09-01T18:49:38Z
dc.date.available 2017-09-01T18:49:38Z
dc.date.copyright 2017-09
dc.date.created 2017-08-23
dc.date.issued 2017-09-01
dc.identifier.uri http://hdl.handle.net/10214/11495
dc.description.abstract Antibiotic-resistant infections are medically concerning due to their difficulty to control and associated rates of mortality. Methicillin-resistant Staphylococcus aureus (MRSA) is a drug-resistant microbe and a prominent cause of skin wound infections. Protegrin-1 (PG-1) is an antimicrobial peptide with a broad spectrum of activity against bacteria, including antibiotic-resistant strains, and therefore possesses promising therapeutic potential. A previous study demonstrated that PG-1 successfully reduced inflammation in mice with chemically-induced colitis. The current study sought to generate proform PG-1 (ProPG) for more efficient activation at sites of tissue inflammation using a recombinant approach. An MMP cleavage site was introduced into ProPG to allow for the more efficient release of mature PG-1 during skin inflammation. The expression vector was constructed and introduced into expression host Pichia pastoris. Fermentation parameters were optimized and efficient ProPG secretion was confirmed by Western blot. Future studies will verify ProPG therapeutic potential for combatting MRSA skin infections. en_US
dc.language.iso en en_US
dc.subject protegrin-1 en_US
dc.subject antimicrobial peptide en_US
dc.subject recombinant protein en_US
dc.subject Pichia pastoris en_US
dc.subject matrix metalloproteinase en_US
dc.subject skin wound en_US
dc.subject methicillin-resistant Staphylococcus aureus en_US
dc.subject MRSA en_US
dc.subject healing en_US
dc.title Production of protegrin-1 with a matrix metalloproteinase/elastase cleavage site and its therapeutic potential for skin wound infections en_US
dc.type Thesis en_US
dc.degree.programme Animal and Poultry Science en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Animal Biosciences en_US
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