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Autophagy Gene Overexpression in Saccharomyces cerevisiae for Accelerated Sparkling Wine Production

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dc.contributor.advisor van der Merwe, George
dc.contributor.author Preiss, Richard
dc.date.accessioned 2017-05-12T13:51:11Z
dc.date.available 2017-05-12T13:51:11Z
dc.date.copyright 2017-05
dc.date.created 2017-04-26
dc.date.issued 2017-05-12
dc.identifier.uri http://hdl.handle.net/10214/10469
dc.description.abstract Traditional sparkling wines are the product of carbonation and aging of a base wine with yeast in the bottle, where yeast cell compounds are released over time which contribute to flavour and appearance. This often lengthy process is known as autolysis. Our lab identified several proteins related to autophagy that result in autolysis when overexpressed. Overexpression of autophagy-related genes ATG3 and ATG4 in industrial wine yeast was found to be a suitable strategy to accelerate cell death and autolysis of wine yeast during nitrogen starvation. We also found that that ATG3 and ATG4 overexpression has pleiotropic ramifications: reduced turnover of autophagic cargo, vacuolar fragmentation, abnormal accumulation of lipids, and accelerated generation of ROS, all of which precede cell death and likely contribute to the impaired response to nitrogen stress. These results work toward solving an important industrial problem, as well as furthering our understanding of autophagy regulation during starvation. en_US
dc.language.iso en en_US
dc.subject autophagy en_US
dc.subject wine en_US
dc.subject autolysis en_US
dc.title Autophagy Gene Overexpression in Saccharomyces cerevisiae for Accelerated Sparkling Wine Production en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US


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