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Analysis of the genetic diversity of Grapevine rupestris stem pitting-associated virus in Ontarian vineyards and construction of a full-length infectious clone

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dc.contributor.advisor Meng, Baozhong
dc.contributor.author Hooker, Julia Catherine
dc.date.accessioned 2017-04-13T20:21:10Z
dc.date.available 2017-04-13T20:21:10Z
dc.date.copyright 2017-04
dc.date.created 2017-03-14
dc.date.issued 2017-04-13
dc.identifier.uri http://hdl.handle.net/10214/10305
dc.description.abstract Grapevine rupestris stem pitting-associated virus (GRSPaV; Betaflexiviridae, Foveavirus) has been associated with a number of diseases including Syrah decline. Previous findings show GRSPaV sequence variants cluster into three or more main phylogroups, regardless of geographical location. Here, the genetic diversity of GRSPaV isolates from Ontarian vineyards was analyzed using broad-spectrum primers targeting the viral polymerase coding sequence. It was hypothesized that GRSPaV variants in Ontario are diverse and GRSPaV-SY variants are involved in Syrah decline symptoms. In total, 169 cDNA clones from 21 Vitis sources were used for phylogenetic analysis. Similarly to previous reports, four major lineages were observed; GRSPaV-PN, -SG1, -SY, and –GG. Variants of the GRSPaV–SY lineage were confirmed in all 14 sources tested with SY-specific primers. Syrah cultivars expressing red canopy decline symptoms had more clones clustering with the GRSPaV-SY lineage than those without observable decline symptoms. GRSPaV clones from 8 hybrid sources mostly clustered with GRSPaV-SY, followed distantly by GRSPaV-BS. A full-length infectious cDNA clone of a GRSPaV-SY-related variant is being constructed to further investigate this relationship. en_US
dc.description.sponsorship NSERC Engage, NSERC Discovery en_US
dc.language.iso en en_US
dc.subject GRSPaV en_US
dc.subject Grapevine rupestris stem pitting-associated virus en_US
dc.subject Syrah decline en_US
dc.subject Infectious clone en_US
dc.subject Genetic diversity en_US
dc.title Analysis of the genetic diversity of Grapevine rupestris stem pitting-associated virus in Ontarian vineyards and construction of a full-length infectious clone en_US
dc.type Thesis en_US
dc.degree.programme Molecular and Cellular Biology en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Molecular and Cellular Biology en_US
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