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Comparison of Platelet Storage Lesions between Leukoreduced and Non-leukoreduced Canine Platelet Concentrates

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Title: Comparison of Platelet Storage Lesions between Leukoreduced and Non-leukoreduced Canine Platelet Concentrates
Author: Oscos-Snowball, Marja A.
Department: Department of Pathobiology
Program: Pathobiology
Advisor: Wood, Darren
Abstract: Background and objectives: Platelet storage lesions (PSL) are the sum of deleterious changes that platelets undergo during storage, affecting platelet concentrate viability for transfusion. In veterinary medicine, transfusion medicine has gained popularity and recent advances have been made. There is a continuous effort to prolong the shelf life and improve the quality of platelet concentrates. Removal of leukocytes has been proven to reduce febrile post-transfusion reactions and decrease platelet premature activation and apoptosis in humans. The present study objectives were to determine if leukoreduced canine platelet concentrates have fewer and delayed onset of in vitro storage lesions compared with non-leukoreduced concentrates and to determine which variable best predicts in vitro platelet viability. Methods: Platelet concentrates from twelve dogs were stored between 22°C – 24°C under continuous agitation for 7 days. Each sample was equally divided into leukoreduced (LR), leukoreduced control (LR-C), non-leukoreduced (NLR) and non-leukoreduced control (NLR-C) concentrates. Platelet count, platelet indices, platelet morphology; pH, glucose, HCO3, pO2, pCO2, and lactate levels; platelet aggregation, and P-selectin and Annexin V expression were assessed on days 1, 3, 5 and 7. All samples were sent for aerobic bacterial culture on day 7. Results: All bacterial cultures were negative and platelet counts did not change significantly over time. There was an earlier onset of decreased pH in NLR samples compared with LR but both decreased over time. All the other variables showed greater deleterious changes in NLR samples compared with LR samples except for platelet morphology and platelet aggregation, which did not have significant changes between LR and NLR, concentrates. No parameter clearly predicted platelet viability. Conclusion: The changes overtime were greater in NLR concentrates than in LR samples. These findings may correlate with a longer shelf life and better in vivo recovery and function of platelets in LR samples compared to NLR samples, but an in vivo study would be needed to confirm this. No single parameter was identified as a predictor of functional shelf life, but leukoreduction clearly improved platelet concentrate in vitro viability.
Date: 2016-12
Rights: Attribution-NonCommercial-NoDerivs 2.5 Canada

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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada