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Assessing temporal changes in macroinvertebrate community structure in a remote wetland through DNA metabarcoding

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dc.contributor.advisor Hajibabaei, Mehrdad Wright, Michael TG 2017-01-06T18:29:01Z 2017-09-19T05:00:21Z 2016-10 2016-09-19 2017-01-06
dc.description.abstract This thesis investigates the use of DNA metabarcoding as a tool for assessing biodiversity patterns in freshwater macroinvertebrate communities within the Peace-Athabasca Delta. Macroinvertebrate samples were collected in June and August of 2012 and 2013 from both the Peace and Athabasca River deltas in northern Alberta. Following the morphological identification of a subset of individuals within the samples, macroinvertebrates were homogenized, bulk DNA was extracted, and two fragments of the mitochondrial Cytochrome c oxidase I (COI) gene were sequenced and compared against two reference libraries obtained 18 months apart. When comparing taxa identified via DNA metabarcoding to those identified through morphology, 73% of the total occurrences were the same. This number increased to 84% through passive additions to online genomic repositories over the course of this study, emphasizing the importance of reference library coverage in metabarcoding analysis. Despite some taxa known to be present in the samples by morphological identification not being identified by DNA metabarcoding, general diversity patterns were consistent between the two methods of identification. The river delta of origin was the primary factor separating macroinvertebrate communities, followed by season of sampling. en_US
dc.language.iso en en_US
dc.rights Attribution-ShareAlike 2.5 Canada *
dc.rights.uri *
dc.subject DNA metabarcoding en_US
dc.subject wetland en_US
dc.subject macroinvertebrate en_US
dc.title Assessing temporal changes in macroinvertebrate community structure in a remote wetland through DNA metabarcoding en_US
dc.type Dataset en_US
dc.type Thesis en_US Integrative Biology en_US Master of Science en_US Department of Integrative Biology en_US

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Attribution-ShareAlike 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-ShareAlike 2.5 Canada