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Investigating and characterizing phoenixin and tachykinin receptor-3 in the reproductive axis of the chicken, Gallus gallus

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dc.contributor.advisor Bédécarrats, Grégoy
dc.contributor.author Sparling, Brandi
dc.date.accessioned 2017-01-06T18:11:15Z
dc.date.available 2017-01-06T18:11:15Z
dc.date.copyright 2016
dc.date.created 2015-12-22
dc.date.issued 2017-01-06
dc.identifier.uri http://hdl.handle.net/10214/10184
dc.description.abstract This thesis identifies two new players, phoenixin and tackykinin receptor-3, in the reproductive axis of the chicken. Phoenixin is known to upregulate pituitary gonadotropin-releasing hormone receptor (GnRH-R) messenger RNA (mRNA) and tachykinin receptor-3 to generate GnRH pulsatility in mammals. Through database search, we confirm that these neuroproteins are encoded in the chicken genome. The full-length mRNA sequences were identified by 5’ and 3’ rapid-amplification of complementary DNA ends and annotated. Phoenixin mRNA was detected and quantified in various tissues via conventional and semi-quantitative PCR, respectively (in diencephalon, pituitary, ovary, gut and heart). In the diencephalon and pituitary of different strain, phoenixin was differentially expressed with age. Tackykinin receptor-3 mRNA is differentially expressed across tissues (diencephalon>pituitary>ovary) and in an age-dependent manner in the pituitary. The function of phoenixin was explored through treatment of sexually immature primary chicken pituitary cells and, unlike rodents no independent modulation of GnRH-R-III/I was detected. en_US
dc.language.iso en en_US
dc.subject Chicken en_US
dc.subject Phoenixin en_US
dc.subject Tachykinin receptor-3 en_US
dc.subject mRNA en_US
dc.subject Reproduction en_US
dc.subject KNDy en_US
dc.title Investigating and characterizing phoenixin and tachykinin receptor-3 in the reproductive axis of the chicken, Gallus gallus en_US
dc.type Thesis en_US
dc.degree.programme Animal and Poultry Science en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Animal Biosciences en_US
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