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Proteomic approaches to early diagnosis of Johne’s disease in dairy cows

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dc.contributor.advisor Dr. Kirby, Gordon
dc.contributor.author Karuppusamy, Shanmugasundaram
dc.date.accessioned 2017-01-05T19:51:01Z
dc.date.available 2017-12-13T06:00:17Z
dc.date.copyright 2016-12
dc.date.created 2016-12-14
dc.date.issued 2017-01-05
dc.identifier.uri http://hdl.handle.net/10214/10172
dc.description.abstract Mycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of paratuberculosis or Johne’s disease (JD), an infectious, chronic granulomatous enteritis of ruminants. Current diagnostic tests for JD have limited sensitivity and specificity for the identification of subclinically infected animals warranting an investigation of MAP species-specific and sensitive antigens that might improve diagnostic test sensitivity and specificity. The aim of the first study was to identify antigenic proteins from the MAP cell envelope by comparing protein profiles from MAP, Mycobacterium avium subspecies hominissuis and M. smegmatis using a 2D-DIGE proteomic approach. Thirteen protein spots were selected and 15 proteins were subsequently identified by LC-MS/MS. The aim of the second study was to generate antibodies to recombinantly expressed MAP cell envelope proteins as well as to an extract of MAP total cell envelope proteins that was subsequently used to identify MAP organisms by IHC and immunomagnetic separation. Six MAP cell envelope proteins were recombinantly expressed, three of which were suitable for polyclonal antibody generation. Polyclonal antibodies generated to an extract of MAP cell envelope proteins detected MAP organisms in infected tissues using IHC and IF techniques thereby providing a more sensitive alternative to acid-fast staining of MAP in tissues for JD diagnosis. Furthermore, these antibodies were effective in immunomagnetic capture of MAP microorganisms in solution thereby providing proof-in-principle for a novel diagnostic approach. The objective of third study was to use an extract of MAP total cell envelope proteins as well as the six recombinant MAP proteins in ELISA formats to detect MAP-specific antibodies in cattle serum. The diagnostic sensitivity and specificity of the MAP envelope protein ELISA after serum absorption was 75% and 96% respectively. While ELISAs using the six recombinant protein antigens had reasonably high sensitivities, specificities were comparatively less than the commercial serum ELISA kit. The potential use of these recombinant proteins ELISAs for diagnosis of early MAP infection and control of JD requires further investigation and validation. en_US
dc.language.iso en en_US
dc.rights Attribution-NonCommercial-NoDerivs 2.5 Canada *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/2.5/ca/ *
dc.subject Proteomics en_US
dc.title Proteomic approaches to early diagnosis of Johne’s disease in dairy cows en_US
dc.type Thesis en_US
dc.degree.programme Biomedical Sciences en_US
dc.degree.name Doctor of Philosophy en_US
dc.degree.department Department of Biomedical Sciences en_US
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Attribution-NonCommercial-NoDerivs 2.5 Canada Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 2.5 Canada