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Apple pomace polyphenols and their effect on the proliferation of human epithelial colorectal adenocarcinoma cells (HT-29)

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dc.contributor.advisor Osborne, Vern
dc.contributor.author Djukic, Vanja
dc.date.accessioned 2016-09-29T20:12:14Z
dc.date.available 2016-09-29T20:12:14Z
dc.date.copyright 2016-09
dc.date.created 2016-09-28
dc.date.issued 2016-09-29
dc.identifier.uri http://hdl.handle.net/10214/10041
dc.description.abstract In an effort to eventually produce a human health promoting, polyphenol-enriched dairy product for human consumption by apple pomace (AP) inclusion in the diet of a dairy cow, two preliminary studies were conducted. Initially, 8 commonly found polyphenols, in locally-sourced AP, were quantified via UPLC/MS and total polyphenol content was determined by the Fast Blue BB salt method. To determine the effect of the rumen environment on the recovery of polyphenols originally found in AP, the sample was subjected to 24hr rumen digestion in an in vitro incubator and the quantities of the polyphenols in the digesta were analyzed by UPLC/MS. The second study evaluated the effect of the aglycone (quercetin) of the most adundant polyphenol in this sample of AP, quercetin-3-glucoside, on the proliferation of human epithelial colorectal adenocarcinoma cells (HT-29). Their proliferation was quantified by use of a cell-protein binding dye, sulforhodamine B. en_US
dc.description.sponsorship Ontario Centres for Excellence, Dairy Farmers of Ontario, Natural Sciences and Engineering Research Council of Canada en_US
dc.language.iso en en_US
dc.subject polyphenols en_US
dc.subject flavonoids en_US
dc.subject colorectal cancer en_US
dc.subject phenolic en_US
dc.subject rumen en_US
dc.subject ruminant en_US
dc.subject dairy cattle en_US
dc.subject HT-29 en_US
dc.subject cancer en_US
dc.subject apple en_US
dc.subject apple pomace en_US
dc.title Apple pomace polyphenols and their effect on the proliferation of human epithelial colorectal adenocarcinoma cells (HT-29) en_US
dc.type Thesis en_US
dc.degree.programme Animal and Poultry Science en_US
dc.degree.name Master of Science en_US
dc.degree.department Department of Animal Biosciences en_US
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