dc.contributor.advisor |
Merrill, Rod |
|
dc.contributor.author |
Poole, Amanda
|
|
dc.date.accessioned |
2016-09-15T20:25:34Z |
|
dc.date.available |
2016-09-15T20:25:34Z |
|
dc.date.copyright |
2016-09 |
|
dc.date.created |
2016-09-13 |
|
dc.date.issued |
2016-09-15 |
|
dc.identifier.uri |
http://hdl.handle.net/10214/10017 |
|
dc.description.abstract |
Pathogenic bacteria cause many human infections, and most employ virulence factors that cause cell or tissue damage in the host. One important virulence factor group is the mono-ADP-ribosyltransferase (mART) family. Vibrio parahaemolyticus is a pathogenic bacterium that encodes a virulence factor, VopT, a mART toxin. VopT modifies Ras, a small GTPase involved in cell signaling, leading to intestinal epithelial tissue damage. VopT is classified as an ExoS-like toxin as it requires the binding of a 14-3-3 protein for activation and shares high sequence identity to other ExoS-like members. Glycohydrolase activities were assessed for wild-type and catalytic variants, the first inhibitors of VopT were identified with IC50 values between 4 and 45 µM, and an ExoT homology model was produced that provides insight into the FAS-dependent activity of VopT. Characterization of VopT has increased our understanding of mART enzymes and may lead to the development of effective therapeutic compounds. |
en_US |
dc.description.sponsorship |
NSERC and CIHR |
en_US |
dc.language.iso |
en |
en_US |
dc.rights |
Attribution-NonCommercial-ShareAlike 2.5 Canada |
* |
dc.rights.uri |
http://creativecommons.org/licenses/by-nc-sa/2.5/ca/ |
* |
dc.subject |
mART toxin |
en_US |
dc.subject |
Vibrio parahaemolyticus |
en_US |
dc.subject |
VopT |
en_US |
dc.subject |
enzyme kinetics |
en_US |
dc.subject |
virulence factor |
en_US |
dc.title |
Kinetic characterization of VopT, a mono-ADP-ribosyltransferase toxin from Vibrio parahaemolyticus |
en_US |
dc.type |
Thesis |
en_US |
dc.degree.programme |
Molecular and Cellular Biology |
en_US |
dc.degree.name |
Master of Science |
en_US |
dc.degree.department |
Department of Molecular and Cellular Biology |
en_US |
dc.rights.license |
All items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated. |
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