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Kinetic characterization of VopT, a mono-ADP-ribosyltransferase toxin from Vibrio parahaemolyticus

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Title: Kinetic characterization of VopT, a mono-ADP-ribosyltransferase toxin from Vibrio parahaemolyticus
Author: Poole, Amanda
Department: Department of Molecular and Cellular Biology
Program: Molecular and Cellular Biology
Advisor: Merrill, Rod
Abstract: Pathogenic bacteria cause many human infections, and most employ virulence factors that cause cell or tissue damage in the host. One important virulence factor group is the mono-ADP-ribosyltransferase (mART) family. Vibrio parahaemolyticus is a pathogenic bacterium that encodes a virulence factor, VopT, a mART toxin. VopT modifies Ras, a small GTPase involved in cell signaling, leading to intestinal epithelial tissue damage. VopT is classified as an ExoS-like toxin as it requires the binding of a 14-3-3 protein for activation and shares high sequence identity to other ExoS-like members. Glycohydrolase activities were assessed for wild-type and catalytic variants, the first inhibitors of VopT were identified with IC50 values between 4 and 45 µM, and an ExoT homology model was produced that provides insight into the FAS-dependent activity of VopT. Characterization of VopT has increased our understanding of mART enzymes and may lead to the development of effective therapeutic compounds.
URI: http://hdl.handle.net/10214/10017
Date: 2016-09-15


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