The effects of delta-9 tetrahydrocannabinol (THC) on granulosa cell viability, apoptosis, and gene expression

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dc.contributor.advisorFavetta, Laura
dc.contributor.authorDufour, Jaustin
dc.date.accessioned2022-08-05T16:53:46Z
dc.date.available2022-08-05T16:53:46Z
dc.date.copyright2022-07
dc.date.created2022-07-28
dc.degree.departmentDepartment of Biomedical Sciencesen_US
dc.degree.grantorUniversity of Guelphen
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeBiomedical Sciencesen_US
dc.description.abstractThis thesis investigates the effects of the psychoactive component of cannabis, delta-9 tetrahydrocannabinol (THC) on bovine in vitro granulosa cell viability, apoptosis, and stress response pathway. 11βHSD1-2 convert cortisol into active and inactive forms. Upon cortisol activation, the glucocorticoid receptor (GR) translocates to the nucleus to regulate transcription. MAPK8-9 enhance GR nuclear export and terminate GR-mediated transcription. Heat shock protein 70 and 90 (HSP70, HSP90) are involved in both THC and cortisol pathways. This study hypothesized that THC counteracts cortisol’s effects by modulating 11βHSDs, increasing GR nuclear export and oversaturating HSPs. Cell viability and apoptosis was assessed in response to THC at clinically relevant doses. Gene expression was analyzed in response to THC and cortisol treatments during culture. The experimental data revealed a potential ability for THC to reduce cortisol signalling in GCs. No effect of THC on cell viability and apoptosis was revealed.en_US
dc.identifier.urihttps://hdl.handle.net/10214/27072
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectstressen_US
dc.subjectcannabisen_US
dc.subjectthcen_US
dc.subjectfertilityen_US
dc.subjectgranulosa cellen_US
dc.titleThe effects of delta-9 tetrahydrocannabinol (THC) on granulosa cell viability, apoptosis, and gene expressionen_US
dc.typeThesisen_US
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