Tracking Changes in a Simulated Human Gastrointestinal Environment following the Introduction of High and Low Virulence Salmonella enterica isolates

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dc.contributor.advisorGoodridge, Lawrence
dc.contributor.authorSimon, Ceylon
dc.date.accessioned2022-12-22T18:06:04Z
dc.date.available2022-12-22T18:06:04Z
dc.date.copyright2022-11
dc.date.created2022-11-25
dc.degree.departmentDepartment of Food Scienceen_US
dc.degree.grantorUniversity of Guelphen
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeFood Scienceen_US
dc.description.abstractOver 2,500 serotypes of Salmonella enterica subspecies enterica exist, however research has focused on epidemiologically relevant serotypes. Changes in a Simulator of the Human Intestinal Microbial Ecosystem (SHIME) model mimicking a healthy human adult were assessed following the introduction of one high virulence (S. Heidelberg (S428)) and one low virulence (S. Falkensee (S37)) S. enterica isolate. Differences in isolate survival, microbial community and metabolic composition, and prophage induction in the gastrointestinal model were analyzed. S. Heidelberg (S428) had enhanced survival and a greater impact on the gastrointestinal metabolome compared to S. Falkensee (S37). Both isolates caused a significant shift in the beta-diversity of proximal colon samples compared to the control. Potential prophage induction was found early (0 h and 6 h) in the treatment period. Community shifts in response to high and low virulence S. enterica strains in the gastrointestinal environment may therefore contribute to their epidemiological importance.en_US
dc.description.sponsorshipCanada First Research Excellence Fund
dc.description.sponsorshipFood From Thought
dc.identifier.urihttps://hdl.handle.net/10214/27350
dc.language.isoenen_US
dc.publisherUniversity of Guelphen
dc.rightsAttribution-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-sa/4.0/*
dc.subjectSalmonellaen_US
dc.subjectS. entericaen_US
dc.subjectSHIMEen_US
dc.subjectvirulenceen_US
dc.subjectserotypeen_US
dc.subjectsurvivalen_US
dc.subjectmicrobial communityen_US
dc.subjectmetaboliteen_US
dc.subjectmetabolomicsen_US
dc.subjectmicrobiomeen_US
dc.subjectguten_US
dc.subjectgastrointestinalen_US
dc.subjectcommunity shiftsen_US
dc.subjectprophagesen_US
dc.subjectphageen_US
dc.subjectprophage inductionen_US
dc.subjectmetagenomicsen_US
dc.subjectgut modelen_US
dc.titleTracking Changes in a Simulated Human Gastrointestinal Environment following the Introduction of High and Low Virulence Salmonella enterica isolatesen_US
dc.typeThesisen
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