Genetic Regulation of Immune Responses in Holstein Dairy Cows across Canada

dc.contributor.advisorMallard, Bonnie
dc.contributor.authorThompson Crispi, Kathleen Adele
dc.date.accessioned2012-09-05T16:30:03Z
dc.date.available2012-09-05T16:30:03Z
dc.date.copyright2012-08
dc.date.created2012-08-09
dc.date.issued2012-09-05
dc.degree.departmentDepartment of Pathobiologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameDoctor of Philosophyen_US
dc.degree.programmePathobiologyen_US
dc.description.abstractDiseases that affect dairy cattle have serious economic and animal welfare implications. The inclusion of immune response (IR) traits in breeding indices has been suggested to improve inherent animal health, and decrease the use of antimicrobials. The objectives of this research were to (1) evaluate cell-mediated (CMIR) and antibody-mediated immune responses (AMIR) on 680 Holstein cows from 58 herds across Canada, (2) estimate genetic parameters of these traits (3) examine the associations with routinely evaluated traits as well as the incidence of mastitis, (4) evaluate the correlation of natural and specific antibody and (5) perform a genome-wide association study (GWAS) to determine genetic markers associated with high or low IR. In collaboration with the Canadian Bovine Mastitis Research Network cows were immunized with both a type 1 and type 2 test antigen to stimulate CMIR and AMIR, respectively. A cutaneous delayed-type hypersensitivity test to the type 1 test antigen was used as an indicator of CMIR, and serum antibody (IgG1 and IgG2) to the type 2 test antigen as an indicator of AMIR. IR phenotypes varied significantly by cow, herd and region in Canada. Heritability estimates were moderate, 0.19 for CMIR and 0.16-0.43 for AMIR depending on time in the immunization protocol and antibody isotype. Beneficial associations between AMIR and some reproductive traits were found. Using estimated breeding values, cows were classified as high, average or low responders. High AMIR cows had significantly lower incidence rates of clinical mastitis compared to average and low cows. No difference was found when cows were classified based on CMIR. Natural antibody was not genetically correlated with specific antibody nor was it associated with mastitis. The GWAS found 198 genetic markers significantly associated with AMIR, with the majority on chromosome 23 where the major histocompatability complex is located. Other significant genes involved in IR include those associated with the complement system, interleukin 17 and tumor necrosis factor. This research confirms the benefit of identifying high IR cows and gives a glimpse of current IR profiles in Canadian Holsteins. This was the first GWAS for IR traits in dairy cattle and suggests it may be possible to include IR traits in genomic selection indices.en_US
dc.description.sponsorshipNational Sciences and Engineering Research Council of Canada
dc.description.sponsorshipAlberta Milk
dc.description.sponsorshipDairy Farmers of New Brunswick, Nova Scotia, Ontario and Prince Edward Island
dc.description.sponsorshipNovalait Inc.
dc.description.sponsorshipDairy Farmers of Canada
dc.description.sponsorshipDairyGen council of Canadian Dairy Network
dc.description.sponsorshipAgriculture and Agri-Food Canada
dc.description.sponsorshipPublic Health Agency of Canada
dc.description.sponsorshipTechnology PEI Inc.
dc.description.sponsorshipUniversite de Montreal
dc.description.sponsorshipUniversity of Prince Edward Island
dc.description.sponsorshipCanadian Bovine Mastitis Research Network
dc.description.sponsorshipDairy Farmers of Ontario Doctoral Research Assistantship
dc.identifier.urihttp://hdl.handle.net/10214/3915
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectImmune responseen_US
dc.subjectDairy cattleen_US
dc.subjectAntibodyen_US
dc.subjectDelayed-type hypersensitivityen_US
dc.subjectIsotype biasen_US
dc.subjectHealthen_US
dc.subjectMastitisen_US
dc.subjectGenome-wide association studyen_US
dc.subjectGenetic parametersen_US
dc.titleGenetic Regulation of Immune Responses in Holstein Dairy Cows across Canadaen_US
dc.typeThesisen_US

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