Effect of whey protein fermentation and hydrolysis on Listeria monocytogenes virulence gene expression in the presence of the gut microbiota modelled in the Simulator of the Human Intestinal Microbial Ecosystem

Simple item page
dc.contributor.advisorLaPointe, Gisele
dc.contributor.authorLi, Grace
dc.date.accessioned2022-09-15T13:54:05Z
dc.date.available2022-09-15T13:54:05Z
dc.date.copyright2022-09
dc.date.created2022-08-31
dc.degree.departmentDepartment of Food Scienceen_US
dc.degree.grantorUniversity of Guelphen
dc.degree.nameMaster of Scienceen_US
dc.degree.programmeFood Scienceen_US
dc.description.abstractVirulence gene expression of the foodborne pathogen L. monocytogenes promotes invasion and spreading within a susceptible host, such as populations with weakened immunity, and can be modulated by protein. In this thesis, enzymatically hydrolysed proteins were shown to reduce actA expression of L. monocytogenes regardless of the source, whereas actA expression was increased by intact whey protein isolate (WPI) but unaffected by individual BCAA and fermented WPI. In simulated gastrointestinal conditions, F2365 showed poor acid tolerance compared to 16PF0833 and ILSI #39, whereas survival and gene expression was similar for the three strains in response to bile and the PC metabolome in the presence of WPI, hydrolysed WPI, or casamino acids. Regardless of the strain and protein treatment, expression was induced for the virulence gene regulator prfA and internalins. The low expression of late-stage virulence genes (hly, plcA, actA) during gastrointestinal stress suggests low PrfA protein activity in these conditions.en_US
dc.identifier.urihttps://hdl.handle.net/10214/27243
dc.language.isoenen_US
dc.publisherUniversity of Guelphen
dc.rightsAttribution-NoDerivatives 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nd/4.0/*
dc.subjectListeria monocytogenesen_US
dc.subjectvirulenceen_US
dc.subjectgene expressionen_US
dc.titleEffect of whey protein fermentation and hydrolysis on Listeria monocytogenes virulence gene expression in the presence of the gut microbiota modelled in the Simulator of the Human Intestinal Microbial Ecosystemen_US
dc.typeThesisen
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Li_Grace_202209_MSc.pdf
Size:
4.65 MB
Format:
Adobe Portable Document Format
Description:
Master's thesis
Download
Collections
Theses & Dissertations - All (2011-
Theses & Dissertations - Harvested by LAC