The structural characterization of Campylobacter jejuni serotype HS: 4 capsular polysaccharide

Loading...
Thumbnail Image

Date

Authors

Chen, Yu-Han

Journal Title

Journal ISSN

Volume Title

Publisher

University of Guelph

Abstract

Campylobacter jejuni' infections are a leading cause of gastroenteritis in humans and usually lead to more severe neurological disorders such as Guillain-Barre? and Miller-Fisher syndromes. The capsular polysaccharides of 'C. jejuni' are thought to play a role in disease pathogenesis, and antibodies to the capsular entities have been detected in humans. In this research, the full structure of 'C. jejuni' serotype HS:4 (strain CG8486) was studied in order to employ it in a multivalent anti-'C. jejuni' vaccine. The structure of capsular polysaccharide of serotype HS:4 was characterized by a series of chemical, analytical, spectrometric and spectroscopic techniques. It consists of a disaccharide repeating unit composed of 3-substituted [beta]-6-deoxy-D-'ido'-heptose and 4-substituted [beta]-D-'N'-acetyl glucosamine with a terminal [beta]-D-'N'-acetyl glucosamine branch and phosphoramidate groups as a structural moiety. This 6-deoxy-heptose constituent is the first characterization with unusual D-' ido' configuration in microorganism.

Description

Keywords

Campylobacter jejuni, infections, gastroenteritis, neurological disorders, capsular polysaccharides, disease pathogenesis, antibodies, C. jejuni serotype HS:4 (strain CG8486), anti-C. jejuni vaccine, serotype HS:4

Citation