Characterization of extended-spectrum cephalosporin-resistant Enterobacterales recovered from dairy manure in Southern Ontario

dc.contributor.advisorBoerlin, Patrick
dc.contributor.authorAnderson, Rebecca
dc.date.accessioned2021-06-10T14:52:32Z
dc.date.available2021-06-10T14:52:32Z
dc.date.copyright2021-06
dc.date.created2021-06-02
dc.degree.departmentDepartment of Pathobiologyen_US
dc.degree.grantorUniversity of Guelphen_US
dc.degree.nameMaster of Scienceen_US
dc.degree.programmePathobiologyen_US
dc.description.abstractFew studies have investigated and characterized extended-spectrum cephalosporin (ESC) resistance among Enterobacterales recovered from dairy manure and even less research has been conducted on ESC-resistance plasmids among Escherichia coli and Klebsiella pneumoniae from livestock in Canada. The objectives of this research focused on the distribution and persistence of ESC-resistant E. coli and K. pneumoniae strains and their ESC-resistance determinants through manure treatment on dairy farms in Southern Ontario. Isolates were recovered from manure samples spanning treatment pipelines on six dairy farms. E. coli and K. pneumoniae carrying blaCTX-M and a subset of E. coli carrying blaCMY underwent whole genome sequencing (WGS) using Illumina short reads, with a subset of these isolates undergoing long read sequencing using Oxford Nanopore Technologies. Results suggest that E. coli is a driving force in the dissemination of ESC-resistance in Enterobacterales in dairy manure and K. pneumoniae also seems important but appears to be less prevalent.en_US
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.description.sponsorshipCIHR
dc.description.sponsorshipJPI-AMR
dc.description.sponsorshipOntario Veterinary College
dc.description.sponsorshipGraduate Student Stipend Support Scholarship
dc.description.sponsorshipQueen Elizabeth II Graduate Student in Science and Technology Scholarship
dc.identifier.urihttps://hdl.handle.net/10214/25906
dc.language.isoenen_US
dc.publisherUniversity of Guelphen_US
dc.rights.licenseAll items in the Atrium are protected by copyright with all rights reserved unless otherwise indicated.
dc.subjectdairy manureen_US
dc.subjectanaerobic digestionen_US
dc.subjectantimicrobial resistanceen_US
dc.subjectEnterobacteralesen_US
dc.subjectCanadaen_US
dc.subjectplasmidsen_US
dc.subjectextended-spectrum cephalosporinsen_US
dc.subjectCTX-Men_US
dc.subjectCMYen_US
dc.titleCharacterization of extended-spectrum cephalosporin-resistant Enterobacterales recovered from dairy manure in Southern Ontarioen_US
dc.typeThesisen_US

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